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ORIGINAL ARTICLE
Year : 2021  |  Volume : 8  |  Issue : 1  |  Page : 52-57

Assessment of oxidative stress in follicular fluid of infertile women undergoing IVF procedure


Ridge IVF Pvt. Ltd, New Delhi, India

Correspondence Address:
Shreya Nautiyal
Ridge IVF Pvt. Ltd, 5/3 Balbir Road, Dalanwala, Dehradun, Uttrakhand-248001
India
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Source of Support: None, Conflict of Interest: None


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Oxidative stress (OS) plays an important role in biological processes of reproduction, and yet there is no accurate indicator to measure it. The objective of the study was to correlate the effect of ROS (reactive oxygen species) on embryo quality and pregnancy outcome. This was achieved by measuring levels of ROS present in granulosa cells and follicular fluid (FF) of women undergoing IVF (in vitro fertilization). ROS in FF is measured using thiobarbituric acid reactive substances assay kit (Abcam) and granulosa cells were measured with the help of flow cytometry. The results showed that high levels of FF malondialdehyde were found in nonpregnant women as compared to pregnant group and were found to be significant (P < 0.0001) and ROS in granulosa cells showed the same trend but was found to be statistically nonsignificant (P = 0.1658). It was also seen that the number of metaphase II oocytes were affected neither by ROS in granulosa cells nor in FF (P > 0.05) but had an impact on the quality of embryos (P < 0.05). Hence, we can conclude that OS is a real threat to embryos especially in vitro and it has a negative impact on IVF outcomes. Imbalance in ROS can lead to poor fertilization rate, implantation, and pregnancy outcomes and ROS levels in FF and granulosa cells can be taken as a potential marker to detect IVF treatment outcomes.


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