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  Indian J Med Microbiol
 

Figure 1 Preparation of intracytoplasmic sperm injection Dish: a 5 µl drop of polyvinylpyrrolidone for priming the injrction pipette. 5 µl HEPES medium drops for keeping the oocytes during ICSI. The number of HEPES drops depends on the number of oocytes to be injected. Everything is covered under oil

Figure 1 Preparation of intracytoplasmic sperm injection Dish: a 5 µl drop of polyvinylpyrrolidone for priming the injrction pipette. 5 µl HEPES medium drops for keeping the oocytes during ICSI. The number of HEPES drops depends on the number of oocytes to be injected. Everything is covered under oil